ABSTRACT
Hesperetin (5,7,3'-trihydroxy-4-methoxyl flavanone) is found in citrus fruits and has antioxidant, anti-inflammatory, anticarcinogenic, antihypertensive and antiatherogenic effects. Acrylamide (AA) has shown neurotoxic and carcinogenic effects in humans with occupational exposures and quantified in staple foods such as coffee, bread, cookies, french fries and in tobacco smoke. In this study, we haveevaluated therapeutic efficacy of hesperetin against AA toxicity. AA was given at 1/3rd of LD50 dose for 10 days to albino rats followed by therapy with different doses of hesperetin for 3 consecutive days. Various toxicity symptoms were observed which include significant reduction of body weight, hair loss, hindlimb splaying, dragging of back legs and irritation on skin. Toxicity symptoms also included significant reduction in level of heamoglobin, GSH, SOD, CAT and significant enhance in AST, ALT, albumin,urea, creatinine, triglyceride, cholesterol with LPO as compared to control group. Activity of acetylcholinesterase was also declined significantly after AA administration, which confirms neurotoxicity. Histopathological observations also supported biochemical studies. Administration of hesperetin at different doses brought the studied parameters towards control in a dose dependent manner concluding its therapeutic effects against acrylamide toxicity in rats.
ABSTRACT
Studies are carried out to remove Fe(II) from wastewater using free and immobilized cells of Pseudomonas fluorescens. Experiments are carried out with free cells between 6 and 8 pH and the uptake of Fe(II) is observed to be maximum at pH 7. Further experiments are done at pH 7. Studies with free and immobilized cells revealed that immobilized cells are more efficient for the removal of Fe(II) than free cells. Fe(II) uptake with Pseudomonas fluorescens is also investigated after the addition of NaCl and MgCl2 to the cells. It is found that the uptake has increased when Sodium chloride (NaCl) and Magnesium Chloride (MgCl2) mixed cells are used. Effect of deficiency of nutrients is also studied. It is found that glucose deficient conditions inhibit Fe(II) uptake more than yeast extract deficient ones. pH also plays an important role in the transport of Fe(II) across the membrane of the cells.